Abstract: Objective: To establish an ultra high performance liquid chromatographic (UPLC) method for determining the lupenone contents in the peel and flesh of Musa nana Lour. and Musa acuminata cv. Mas (AA) harvested at different times and to optimize the extraction conditions of lupenone. Methods: Samples were extracted with methanol by refluxing and the extracts were concentrated under reduced pressure. The analysis was performed on Waters ACQUITY UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm), with acetonitrile:water (85:15, V/V) solution as the mobile phase. The flow rate was 0.3 mL/min, column temperature was 30 ℃ and the detection wavelength was set at 206 nm. Results: No lupenone was detected in the flesh of Musa nana Lour. and Musa acuminata cv. Mas (AA), but high lupenone contents were detected in the peel. A good linearity of the calibration was observed in the range of 16.70?59.96 mg/100 mL (r = 0.999 6). The average recovery of lupenone was 99.00% and the precision expressed as relative standard deviation (RSD) was 3.0%. Conclusion: This method had the advantages of high recovery, good reproducibility, high specificity, high accuracy and simplicity. It was useful for the determination of lupenone in the peel and flesh of Musa nana Lour. and Musa acuminata cv. Mas (AA).

Key words: Musa nana Lour. peel, Musa acuminata cv. Mas (AA). peel, lupenone, UPLC