Abstract: Ultraviolet-visible spectroscopy, infrared spectroscopy and high performance liquid chromatography were applied to explore the compositions of crude and purified polyphenols from “Fertod Zamatos” red raspberry, and the antilipid peroxidation activity of red raspberry polyphenols was evaluated by three in vitro assays. The composition analysis showed the presence of flavonoids, anthocyanins and glycosides in red raspberry polyphenols. Proanthocyanidins B2, rutin, salicylic acid and ellagic acid were detected in red raspberry polyphenols, and there were some differences in the phenolic profile between the crude and purified polyphenols. Red raspberry polyphenols in a certain concentration range had inhibitory effects on Fe2+-induced lipid peroxidation in yolk lipoprotein, H2O2-induced hemolysis of rat red blood cells and spontaneous lipid peroxidation in rat liver tissue homogenate in a concentration-dependent manner. But crude red raspberry polyphenols showed stronger anti-lipid peroxidation activity than the purified one. Therefore, the polyphenol extracts from red raspberry have anti-lipid peroxidation activity; however, further studies are needed to investigate the underlying mechanism and pathways.

Key words: red raspberry, polyphenols, anti-lipid peroxidation, flavonoid, anthocyanins, glycosides