Abstract: This research aimed to analyze the diversity and composition of microbial communities in soils as well as on grapevine leaves and berries from three vineyards located in Xinjiang by high-throughput sequencing. A total of 1 043 102 fungal high-quality sequences were obtained from 27 samples, representing 237 fungal genera in 5 fungal phyla, as well as 2 422 188 high-quality bacterial sequences, representing 8 bacterial phylum and 314 bacteria genus were obtained. The soil samples showed the highest diversity and quantity of both fungi and bacteria followed by grape leaves and berries. The predominant fungi identified in the soil included Saccharomyces, Sordaria, Tetracladium and Geomyces, and the predominant bacteria included Arthrobacter, Kaistobacter and Skermanella. However, for both plant materials, the predominant fungi included Aureobasidium, Cryptococcus, Aspergillus and Sporospora, and the predominant bacteria included Pseudomonas, Sphingomonas and Adhaeribacter. Moreover, microbial diversity varied from vineyard to vineyard. In summary, understanding of the complex microbial communities in the vineyard can offer a theoretical basis for further exploitation and utilization of distinctive microorganisms in the wine industry.

Key words: high-throughput sequencing, microbial communities, diversity, soil, wine grapes, grape leaves