FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (11): 126-131.doi: 10.7506/spkx1002-6630-201811020

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect of Fish Oil on Glucose Metabolism and PI3K/Akt Signaling Pathway in Diabetic KKAy Mice

HU Mingyuan1, WANG Feng1, MA Yongjian1,2, SUN Guiju1,*   

  1. 1. Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China; 2. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China
  • Online:2018-06-15 Published:2018-06-06

Abstract: Objective: To investigate the effect of fish oil on glucose metabolism and phosphatidylinositol 3-kinase (PI3K)/ protein kinase B (Akt) signaling pathway in diabetic KKAy mice. Methods: Forty KKAy mice were randomly assigned into four groups: diabetic model group, low-dose fish oil group, medium-dose fish oil group and high-dose fish oil group. Ten male C57BL/6 mice served as a non-diabetic control group. Blood and tissue samples were collected after 12 weeks of treatment. Blood glucose, serum insulin, adiponectin, leptin, and the mRNA expressions of insulin receptor substrate-1 (IRS-1), PI3K, Akt and glucose transporter-4 (GLUT-4) were detected. Results: Compared with the model group, fasting blood glucose was reduced significantly in the low-dose fish oil group (P < 0.01), fasting insulin was increased in both the low and medium-dose groups (P < 0.05), and adiponectin level was significantly increased in all the treatment groups (P < 0.05). In addition, fish oil at all doses up-regulated the mRNA expression of IRS-1, PI3K, Akt and GLUT-4 and at low dose had more significant effect on the mRNA expression of Akt and GLUT-4. Conclusion: Fish oil at low dose may improve glucose metabolism by regulating the PI3K/Akt signaling pathway.

Key words: type 2 diabetes mellitus, fish oil, glucose metabolism, insulin resistance, PI3K/Akt signaling pathway

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