Abstract: In this study, comparative transcriptomic analysis of Yarrowia lipolytica grown with glucose (control group) and oleic acid (experimental group) as the carbon source was conducted using Illumina high-throughput sequencing and the data were analyzed by various bioinformatics methods. A total of 17 923 921 clean reads were obtained from the sequencing results of the control group, and 22 656 852 clean reads from the sequencing results of the experimental group. A total of 536 significantly differentially expressed genes were obtained between the two samples, of which 376 genes were up-regulated whereas the remaining 160 genes were down-regulated. GO and KEGG enrichment analysis showed these differential genes participated in the regulation of various biological processes. This study provides an important basis for subsequent molecular biology research.

Key words: Yarrowia lipolytica, transcriptome, differentially expressed genes