FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (17): 182-186.doi: 10.7506/spkx1002-6630-201817030

• Nutrition & Hygiene • Previous Articles     Next Articles

Foodborn Salmonella Isolates: Pathogenicity Evaluation and Detection of Virulence Genes

LIU Shuhong, WU Kemin, ZHOU Chenyu, LIANG Jingzhen, WEI Ping*   

  1. Institute for Poultry Science and Health, Guangxi University, Nanning 530004, China
  • Online:2018-09-15 Published:2018-09-18

Abstract: The purpose of this study was to evaluate the pathogenicity of the predominant serotypes of Salmonella isolated from retail fresh meat and the relationship between the pathogenicity and virulence genes. The pathogenicity of the dominant serotypes including S. agona, S. derby, S. enteritidis and S. typhimurium against specific pathogen free (SPF) Kunming mice was evaluated by improved Karber’s method, and the distribution of 9 virulence genes was detected by polymerase chain reaction (PCR). The results demonstrated that four predominant Salmonella serovars showed high mortalities of up to 40%?70%. S. enteritidis, S. agona and S. derby had similar pathogenicity, while weaker pathogenicity was observed for S. typhimurium. The pathogenicity of Salmonella was associated with the sseL gene on Salmonella pathogenicity island (SPI-2).

Key words: foodborn, Salmonella, serotype, pathogenicity, virulence genes

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