Antifungal Action of α-Terpineol on Imazalil-Resistant Penicillium digitatum Pdw03

doi:10.7506/spkx1002-6630-20210110-098

Abstract

Abstract: This study explored the inhibitory effect and potential antifungal mechanism of α-terpineol against an imazalil-resistant strain of Penicillium digitatum Pdw03. In vitro experiment showed that α-terpineol exhibited antifungal activity against P. digitatum Pdw03, with minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of 4.00 μL/mL. In vivo results showed that α-terpineol at MFC and 5 MFC significantly reduced the incidence of green mold and lesion diameter in ponkan fruit inoculated with P. digitatum Pdw03. Propidium iodide (PI) staining indicated that 1/2 MIC α-terpineol evidently decreased the cell membrane integrity, ergosterol content (P < 0.05) and gene expression of squalene synthase (ERG9) (P < 0.05) in P. digitatum Pdw03. These results suggest that α-terpineol affects the growth of P. digitatum Pdw03 by inhibiting the gene expression of ERG9, and thus is an effective alternative for controlling postharvest citrus diseases.

Key words: α-terpineol; Penicillium digitatum Pdw03; cell membrane; ergosterol; ERG gene

CLC Number:

Q939.92

OUYANG Qiuli, LIU Yangmei, CHEN Yue, TAO Nengguo. Antifungal Action of α-Terpineol on Imazalil-Resistant Penicillium digitatum Pdw03[J]. FOOD SCIENCE, 2022, 43(7): 8-13.

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Antifungal Action of α-Terpineol on Imazalil-Resistant Penicillium digitatum Pdw03

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