Study on Assay of γ-Linolenic Acid of Myocastol Coypus by AgNO3-TLC and HPLC

Abstract

Abstract: With saponification and the improved urea inclusion method,the content of polyunsaturated fatty acid(PUFA),especially has been enriched by γ-linolenic acid.An AgNO3-Thin-Layer Chromatography(AgNO3-TLC)method for the isolation of γ-linolenic acid in the content of PRFA was reported and the conditions were:the solvent systems of benzene-methanol-glacial acetic acid was 9+0 4+0 4respecfireh the color developmint reagent was ethanol solusion containing 10%(W/V)molybdophosphoric acid.High Performance Liquid Chromatography (HPLC) was used to determine the γ-linolenic acid.The sample was analyzed on a C18 column with acetonitrile and water used as gradient elution and assayed by 242nm UV detector.γ-Linolenic acid was determined well.

Key words: Myocastor coypus , γ-Linolenic acid, Urea inclusion , AgNO3-TLC, HPLC

MO Xiang-Tao, SHI Hong-Juan, CHEN Yu, LI Xiang-Zhong, XIE Wei-An, XIA Li-Qiu, XU Li-Gui. Study on Assay of γ-Linolenic Acid of Myocastol Coypus by AgNO3-TLC and HPLC[J]. FOOD SCIENCE, 2002, 23(12): 105-109.

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Study on Assay of γ-Linolenic Acid of Myocastol Coypus by AgNO3-TLC and HPLC

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