FOOD SCIENCE ›› 2002, Vol. 23 ›› Issue (2): 100-103.

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Determination of Isoflavone Conten t in Fermented Soybean Food by HPLC

 YIN  Li-Jun, LI  Li-Te, LI  Zai-Gui, CHEN  Si-Ying-San   

  • Online:2002-02-15 Published:2012-02-13

Abstract: The high performance liquid chromatographic determination of isoflavo ne is described.Isoflavone was gradientlyelutedbyacetonitrile -water -aceticacidona C18reversedphasecolumn.Itwas foundt hereis agoodlinear relationship,separation degree an d reproducibility by the simple,fast and accurate method to determine th e isoflavone.The average rateofisoflavoneis morethan99%.The waveis254nm.The contentofdaidzeinandgenis teinis higher thandaidzinand genistininf ermentedsoybean food.

Key words: Isoflavone , Highperformance liquid chromatography