Studies on Solid State Fermentation Conditions of GLALipid fromRhizopus Arrhizus

Abstract

Abstract: AmutantstrainRC378accumulating a large amountof lipidwas successful ly obtainedas treatedby U.V.,He -Ne laser and LiCl to induce compou nd mutagen.The content of lipid in RC378cultivated in shaking flask was47.8%.The content ofGLAinLipidwas12.6%.After studying onlipidprodu cing conditions ofRC378,the optimu m fermentation conditions for lipid p roduction were:Wheat bran∶Corn pow der∶Repeseed residue∶Rice flour =6∶4∶2∶1.Withtheaddingof2%sucrose,0.2%NH 4 Cl,0.4%sodiumcitricacid,and 0.3%ZnSO 4 ,theratioofsubstrateand water was 1∶1.5,inoculatedwithsuspensionspore.Inthefirst72h,itwa s cultivatedat30℃,andintheother60hitwascultivated at24℃.The content of lip idwas kept at9.4%～12.9%,and GLAkep t at9.8%～12.6%.

Key words: GLA , Lipid , Solid state fermentation, Rhizopus arrhizus

WANG Wei-Wei, REN Peng-Kang, YAN Ming, SHI Qiu-Fang. Studies on Solid State Fermentation Conditions of GLALipid fromRhizopus Arrhizus[J]. FOOD SCIENCE, 2002, 23(6): 94-98.

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Studies on Solid State Fermentation Conditions of GLALipid fromRhizopus Arrhizus

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