FOOD SCIENCE ›› 2003, Vol. 24 ›› Issue (10): 97-100.

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Study on Strains Screening for Producing Protopectinase and Its Fermentation Properties

 LU  Zhao-Xin, YOU  Hua, LU Feng-Xia , SUN  Qing-Zhu   

  1. College of Food Science and Technology,Nanjing Agricultural University
  • Online:2003-10-15 Published:2011-12-13

Abstract: A strain XZ3, of high protopectinase activity, was screened out from 24 samples originated differently. Its enzymaticactivity arrived at about 126.4U/ml. The strain was identified as Aspergillus sp. by surveying the colony and sporangiumconformations. Studies on the pH, the content of reducing sugar, the weight of wet mycelium and the PPase and PGase activityof the fermentation system , determined in different time, showed that in the course of fermentation, the volume of reducing sugarincreased quickly in the early stage while decreased quickly when the mycelium was in logarithmic growth stage. The pH keptstable in the fermentation. The change of enzymatic activity was synchronous with the growth of mycelium and the ratio ofPPase and PGase activity kept constant to a certain extent.

Key words: strain, protopectinase, screening, fermentation