FOOD SCIENCE ›› 2003, Vol. 24 ›› Issue (3): 102-105.

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Study on Methods for Determination of Lactic Acid in Fermented Broth

 ZHENG  Zhi, JIANG  Shao-Tong, PAN  Li-Jun   

  • Online:2003-03-15 Published:2011-12-13

Abstract: EDTAtitration is a main method for th e determination of lactic acid in fermented broth.In this paper,factors influencing determination of lactic acid were studied with cont rol experiment and high performance liquid chromatography(HPLC).The results indicated that lactic a cid in fermented,broth must be neutr alized with excessive CaCO 3 before titration,then the pH value of solution was adju sted to exceed12.0with NaOH with calceinas indicator,a good reprodu cible result and recovery rate could be achieved.The levels obtained by HPLC in comparison with EDTA titration showed that EDTAwould yield excessive lactic acid data,but after calibration,EDTA method could still be used for quality control during lactic acid pro cessing.

Key words: lactic acid, EDTA, titration HPLC