Abstract: In this study,compound propolis formulae were prepared from making up th e propolis’ ethanol extract with Chinese herbal broths o f licorice(Radix Glycyrrhizac)roots and herbaceous peony(Paeonialaciflora)roots.By means of the formula,myent eric motion tests were then carried o ut in vitro,In vitro bacteriostatic tests were als o conducted against some enteropath ogens and opportunistic pathogens,such as Shigella flexneri,Salmonella,Staphlococcus aureus,Escherichia coli,Pseudomonas aeruginosa and Proteus.The tests revealed that the formula showed certain inhibitive effect on the peristalsis of in-testinal smooth muscles and also rem arkable antispasmodic and abirrita nt effects.Compared with acetylcholine(Ach)and epinephrine,two neutrotransmi tters and atropine,an antagonist,t he actions of compoundpropolis formula on intestinal smooth muscles have not realize dthroughα-,β-or M-re-ceptors.Instead,they were probabl ymyogenicorviaotherways.Inthein vitrobacteriostatictests,theformula showedstronginhibitiveeffecn on t he bacterial strains tested.All of t heir MICs were lower than500×10 -6 ,while those on Salmonella and Shigella flexneri were both250×10 -6 .The study formed a pharmacological experimental basis for the application of compound propolis formulatio ns as a functional food to regulate digestivefunctions.

Key words: propolis, antispasmodic test, bacte riostatic tests, functional food