Abstract: Rice wine, a traditional local product in Xiaogan of China, contained a great amount of rhizopus strains, rich inmiroorganism enzymes.Is had a flavor of genuine mellowness and slight sweetness.In production,the quality of its strains wasso unstable that the initial isolation and identification of the strains were needed.13 quality strains were screened out of 36 speciesfor the culture of quality yeast so that stable high-quality products could be obtained all the time.

Key words: strain, isolation, identification, screening, cultivation, pure culture, mixture yeast