Abstract: Cholesterol exists in all the tissue of the animals,and it’s essential for the animals to maintain their normal physiologicbehavior.But the surplus cholesterol in human body will leads to high blood cholesterol level and furthermore results in a seriesof vascular disease,so we should control the dietary intake of the cholesterol,and therefore it is greatly necessary to determine thecholesterol concentration in the food product in order to settle a precise determination method of the cholesterol concentration.The colormetric method is used in this paper.The micro-amount of the sample is used in this method,which doesn’t need to extractthe lipid and just uses the direct saponofication method,thus using this method not only avoids using the mosttoxic andexpensive material but also reduces the time of the operation significantly,this method can promote the completion of the wholesaponification proceeding by the addition of nitrogen gas and Boron Trifluoride Etherate.The best saponification conditions arechosen out by the orthogonal experiment.The results measured has a high precision value.The variation coefficient is 1.32%,therecovery ratio which 84.0%～89.7% makes the researchers satisfied.In the paper parts of seafoods are analyzed,except thedetermination of the cholesterol concentration in the egg yolk.The results of the determination denis that there is high cholesterollevel in squids,and shows that the cholesterol nearly exists in the internalorgans of the squids,when people eat them,they needn’tconsider surplus intake of the cholesterol.Finally the consequence of this experiment shows this method also adapt to thedetermination of other samples.

Key words: cholesterol, the colormetric method, the rapid determination method