FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (1): 174-177.

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Comparison of Antiserum by Injecting Balb/c Mouse with Different Coupling Ratio Clenbuterol Immunoconjugates

 PAN  Ke, SUN  Yuan-Ming, HUANG  Li, LEI  Jiang-Tao, YU  Hong-Ying   

  1. l.Food Science College of South China Agricultural University;2.Guangdong Agriculture,Industry and Business Polytechnic College
  • Online:2004-01-15 Published:2011-10-24

Abstract: Immunizing Balb/c mouse with three kinds of clenbuterol lmrmunogens whose coupling ratios are 6.1:1, 13.2:1, 18.9:l respectively, getting the anti-clenbuterol serum and detecting them with indirect ELlSA and indirect competitive ELISA.Theresults show that the antiserurn of CL13.2-BSA is prior to the other two clenbuterol immunogens, which has a higher titer andsensitivity, the linear detection scope was 1~10μg/ml.

Key words: clenbuterol, coupling ratio, antiserum, Balb/c mouse, ELISA