FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (4): 150-152.

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Determination of Chlorogenic Acid of Nanguoli Pear by HPLC

 XIN  Guang, LIU  Chang-Jiang, HOU  Dong-Yan, WU  Jun-Qing   

  1. 1.Department of Chemistry,Anshan Normal University;2.College of Food Science, Shenyang Agricultural
  • Online:2004-04-15 Published:2011-10-24

Abstract: Objective: To establish a new HPLC method for determination of chlorogenic acid(CGA)in Nanguoli pear.Method:CGA was analysed by using C18 column(250mm×4.6mm,10μm) with detector at 327nm, and the mobile phase consistingof CH3OH-0.4% H3PO4 (25:75).Results: The recoveries of CGA was 98.5%~102.6%,precision (RSD) was 1.6%~2.1% (n=5).Conclusion:Quantitative analysis showed that the content of CGA in the core peel and pulp of Nanguoli pearwere 1.785, 0.271 and 0.714mg/g respectively.

Key words: HPLC, Nanguoli pear, chlorogenic acid