FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (4): 50-52.

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Screening of a High Polyunsaturated Fatty Acid Yield with Mortierellaisabellina Mutated by UV and LiCl

 WANG  Xiao, QIU  Shu-Yi, HE  La-Ping   

  1. Province Key Laboratory of Fermentation Engineering and Biological Pharmacy,Guizhou University of Technology
  • Online:2004-04-15 Published:2011-10-24

Abstract: A mutant strain MUI0310 was successfully obtained when the original strain Mortierella isabellina AS3.2793 wastreated by UV and LiCl as mutating agent again and again .The biomass was up to 21.80g/L, which was 107.62% higher than thatof the original strain while the content of lipid was up to 61.47%, which was 5.50% higher than that in the original strainmycelium. Analysis by gas chromatograph indicated the content of PUFA in the lipid was 23.21% . After passing on many times,there were no distinct changes on the production. Fermentation test shows MUI0310 has a capability to be improved to industrialstrain.

Key words: Mortierella isabellina, polyunsaturated fatty acid, mutation