Quantitative Determination of Dehydropachymic Acid in Poria cocos by RP-HPLC

Abstract

Abstract: Objective: To determine the content of dehydropachymic acid in Poria cocos. Methods: The RP-HPLC methodwas used to determine the content of dehydropachymic acid in Poria cocos. The analysis was carried out on Phenomenex LunaC18 column(4.6mm×150mm,5μm). The mobile phase was CH3CN-0.05%H3PO4 (64:36, V/V), flow-rate 1.0ml/min, wavelength242nm and temperature 25℃. Results and conclusion: The method was simple and had a good linear relationship. The liner rangewas 0.35～1.75μg and gave a correlation (r) of 0.9991. The recovery was 97.86% and RSD 1.52%. The method was easy todetermine the content of dehydropachymic acid in Poria cocos.

Key words: dehydropachymic acid, Poria cocos, HPLC

YI Zhong-Hong, XU Xiu-Ying, ZHENG Yi-Min, WANG Long. Quantitative Determination of Dehydropachymic Acid in Poria cocos by RP-HPLC[J]. FOOD SCIENCE, 2005, 26(7): 198-199.

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Quantitative Determination of Dehydropachymic Acid in Poria cocos by RP-HPLC

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