Abstract: In older to gain the optimization extract of polysaccharide of Rana chensinensis David by pulsed electric field (PEF), we have inspected single factor influence and then optimized them by orthogonal experiment. The result was showed that the largest extraction ratio is 55.59% by PEF on the conditions of 0.5% KOH and 20kv/cm electric field intensity and 6 μ s pulse number. Compared it with the alkali extract method and enzyme extract method and compound extract method, the extract ratio and polysaccharide content of PEF method are higher than the other three methods. The PEF extract ratio is 1.77 time of compound enzyme method and total sugar contents is more 6.34% than compound enzyme method and the impurity of extraction material is less. So PEF method is a novel and promising method to extract polysaccharide.

Key words: pulsed electric field(PEF), polysaccharide of Rana chensinensis David, orthogonal experiment