Abstract: Objective In order to explore effect on the expression of hormone-sensitive lipase(HSL)gene in white adipose tissue. Methods Fifty male Sprague-dawley(SD) rats were randomly divided into control group and high-fat group and they were fed with basic diet and high-fat diet respectively for 13 weeks. DIO-R and DIO rats were selected according to their body weight and the quantity of energy intake, then observing changing of the body fat content ,the level of blood fat was determined by enzyme method, the level of serum growth hormone was determined by radioimmunoassay and the level of the HSL mRNA in white adipose tissue was determined by RT-PCR. Results The body fat content of DIO-R rats were lower than those of DIO rats (p<0.05). The body fat contents, TG and CHO of DIO-R rats were lower than those of DIO rats(p<0.05),while there was no significant difference between DIO-R and DIO rats in the level of HDL-C(p>0.05).The level of serum growth hormone of DIO-R rats were higher than those of DIO rats(p<0.05). High fat diets increased the level of the HSL mRNA in DIO-R rats. Conclusion High fat diets increased the expression of HSL in white adipose tissue in resisting diet-induced obesity of rats.

Key words: high-fat diet, hormone-sensitive lipase, lipid metabolism