FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (9): 494-497.

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Comparison of Antiserum by Injecting Three Kinds of Mouses with Clenbuterol Immunoconjugate CL13.2-BSA

 HUANG  Li, PAN  Ke, SUN  Yuan-Ming   

  1. 1.Food Science College of SCAU; 2.Guangdong AIB Polytechnic College
  • Online:2005-09-15 Published:2011-09-20

Abstract: Immunizing Balb/c mouse, Kunming mouse, SD rat respectively with Clenbuterol immunogen whose coupling ratio is 13.2:1, getting the anti-Clenbuterol serum and detecting them with indirect ELISA and indirect competitive ELISA. the results show that the antiserum of Kunming mouse is lower than that of Balb/c mouse and SD rat in the matter of titer, specificity and sensitivity; the antiserum of SD rat has a higher titer and sensitivity, the linear detection scope was 0.01ng/ml~1μg/ml,which has the value of development and application with the feature of big blood volume.

Key words: Clenbuterol, immunogen, antiserum, ELISA