Abstract: The optimum liquid culture conditions producing β-glucosidase from Pleurotus tuber regium (Fr.) Sing were studied. The optimum conditions for fermentation were: the soybean milk as the nitrogen source, the glucose as the carbon source, the initial optimum pH was 6.0. Under the optimal incubation condition: culture temperature was 25℃, volume 50ml/250ml, rotate speed 150r/min and culture time 72h, the highest β-glucosidase activity was obtained at wet weight of 1.86U/g (wet mycelium).

Key words: Pleurotus tuber regium (Fr.) Sing； liqiud culture； &beta, -glucosidase；