FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (10): 188-192.

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Studies on the Separation and Purification of IgG in Alaxa Bactrian Camel Milk and Its Heat Stability

 JI  Ri-Mu-Tu,   Zhang-He-Ping,   Zhao-Dian-Bo   

  1. Key Lab of Dairy Biotechnology and Bioengineer, Ministry of Education, College of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot 010018, China
  • Online:2006-10-15 Published:2011-11-16

Abstract: The camel milk IgG was separated and purified by using saturated ammonium sulphate combined with anion exchange resin such as DEAE-Sephacel. The purity and molecular weight of IgG was determined by SDS-PAGE. The specific rabbit anti-camel IgG antiserum was prepared by injection of camel IgG into different sites of rabbits. Camel skim milk samples were heated at 65, 75, 85, 100℃ for 30min, respectively. Presence and identification of IgG were monitored using SDS-PAGE technique and the change of IgG activity was measured by RID method. The whole activity of IgG in reference values of cow milk was lost at 75℃/ 30min versus 68.9% in loss activity of camel milk IgG. Therefore, the heat stability of camel milk IgG was higher than cow milk IgG.

Key words: bactrian camel milk, IgG, heat stability