FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (10): 311-341.

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Study on Wheat Bran Degraded by Trichoderma koningii

 ZHAO  Yu-Ping   

  1. Department of Bioengineering and Chemical Engineering, Huaiyin Institute of Technology, Huaia’n 223001, China
  • Online:2006-10-15 Published:2011-11-16

Abstract: Trichoderma koningii which had been separated was studied in this paper. The conditions of the filter paper activity (FPA) test were studied and analyzed, which were showed as follows: enzymolysis for 40 minutes on the condition of 60℃ and pH4.5, proportion of cellulase to substrate was 1:2. The optimum fermentation conditions were showed as follows: the culture medium was pH6.5, water content was 150%, the amount of inoculation was 5%, tempera- ture was 28℃, fermentation time was 4d. The transformation efficiency could reach 28.5% when the wheat bran had been removed protein and starch.

Key words: wheat bran, cellulase, cellulase activity, transformation efficiency