Determination of Bright Blue in Bitter Gourd Beer by High Performance Liquid Chromatography

FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (12): 657-659.

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Determination of Bright Blue in Bitter Gourd Beer by High Performance Liquid Chromatography

LONG Zhou-Xiong, HU Hai-Shan, WAN Chun-Hua, YAN Bing

Jiangxi Institute of Analysing and Testing, Nanchang 330029, China

Online:2006-12-15 Published:2011-11-23

Abstract

Abstract: An HPLC method was developed to determine bright blue in bitter gourd beer. After being extracted with polyamide resin, bright blue could be adsorbed on Kromasil C18 column, and eluted gradiently by using acetonitrile -0.02mol/L ammonium acetate as the mobile phase at flow rate 1.0ml/min, and be detected at 627nm. The recovery of sample is in the range of 89.6%～ 97.6%, RSD(n=6) is 3.25%. This method is proved to be simple, rapid and accurate .

Key words: HPLC, bitter gourd beer, bright blue

LONG Zhou-Xiong, HU Hai-Shan, WAN Chun-Hua, YAN Bing. Determination of Bright Blue in Bitter Gourd Beer by High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2006, 27(12): 657-659.

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Determination of Bright Blue in Bitter Gourd Beer by High Performance Liquid Chromatography

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