Abstract:  Objective: To observe the immune regulating effect of ginkgo albumin(GAP). Methods: In vivo normal mice, the effectson immune organ,carbongraular clearance ability, hemolysin production level, DTH induced by DNFB were assayed. MTTmethod was employed to investigate T lymphocyte and B lymphocyte proliferation LDH method to investigate the cytotoxic-ity of NK cells, the proliferation of thymus cells method to investigate activity of IL-2, and FACS method to investigate effectof GAP on T lymphocyte subsets. Results: In normal mice, GAP can increase spleen index and thymus index, improvephagocytosing function of peritoneal macrophage, significantly intensify DTH, and promote production of serum hemolysinsimmunized with SRBC. GAP can also promote proliferation of activated and inactivated T lymphocyte`as well as B lymphocyte,increase the cytotoxicity of NK cells and induce secretion of spleen lymphocyte. GAP can increase percent of L3T4+ cells(%),percent of Lyt2+ cells(%)and ratio of L3T4+ cells /Lyt2+ cells. Conclusion: GAP can significantly increase the immune regulatingfunction of normal mice.

Key words: ginkgo albumin (GAP), immunologic function, lymphocyte, peritoneal macrophage, IL-2, T lymphocyte subsets