FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (3): 96-99.

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Improvement Study on Acid and Bile Tolerance of Lactobacillus acidophilus Protoplast Fusion

 WANG  Yu-Hua, ZHANG  Gui-Rong, LIU  Jing-Sheng   

  1. 1.Department of Food Engineering, Jilin Agricultural University, Changchun 130118, China;2.Changchun Institute of Education, Changchun 130046, China
  • Online:2006-03-15 Published:2011-09-06

Abstract: Lactobacillus acidophilus La-w1 and La-w2 from different sources were successfully fused by using the protoplastfusion technique. Fusion hybrid La-F1 was obtained, and its growing characteristics cultivated in skim milk for 48 hours at 37℃ werestudied. Results showed that La-F1 has better acid and bile tolerance than initial strains and the fermented characterics meet therequirement of lactic acid fermented food.

Key words: Lactobacillus acidophilus, protoplast fusion, acid and bile tolerance