FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (4): 71-74.

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Experimental Study on Converting Fumaric Acid to L-malic Acid by Mutated Aspergillus wentii

 JING  Xiao-Hui, DING  You-Tu   

  1. 1.College of Chemistry and Chemical Engineering, Nantong University, Nantong 226007, China; 2.Ferment Factory of Nantong Food Engineering Group Company, Nantong 226001, China
  • Online:2006-04-15 Published:2011-09-13

Abstract: The Aspergillus wentti which had been treated with 60Co was selected as the strain to convert fumaric acid to L-malic acid in 200L fermentor by liquid-submerged fermentation. The effects of culture temperature, nitrogen sources and vibration models of sorting table on the growing state of strain were examined. Fermentative factors such as variations of aeration rate, temperature, pH and rotated speed, that could affect the conversion rate of fumaric acid and production of L-malic acid , were investigated. The results showed that Aspergillus wentti which had been treated with 60Co could not only inhibit the production of by-products but also improve the selectivity of product and shorten the culture time of strain .

Key words: mutation, fumaric acid, L-malic acid, submerged culture