Determination Study on Chloramphenicol Residue in Aquatic Products by Gas Chromatography with Electron Capture Detection (GC-ECD)

Abstract

Abstract: The method for determination of chloramphenicol residue in aquatic products by GC-ECD was to be improved. After extraction with ethyl acetate and air evaporation, the residues were dissolved in 0.01mol/L HCl. The lipid was removed with n- hexane to derive the chloramphenicol with BSTFA+TMCS(99+1) in ethyl acetate by a ultrasonic bath. The derivate was determined by GC and quantified by an external standard. The recoveries are above 85% when added with chloramphenicol in a range of 0.2～2.0μg/kg. The relative standard deviations are between 0.9%～2.7%, and the calibration coefficient is 0.999.

Key words: , aquatic products； chloramphenicol residue； GC(gas chromatography)；

GONG Xiang-Hong, XU Ying-Jiang, ZHANG Xiu-Zhen, LIU Li-Juan. Determination Study on Chloramphenicol Residue in Aquatic Products by Gas Chromatography with Electron Capture Detection (GC-ECD)[J]. FOOD SCIENCE, 2006, 27(7): 222-224.

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Determination Study on Chloramphenicol Residue in Aquatic Products by Gas Chromatography with Electron Capture Detection (GC-ECD)

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