Abstract: Sesamin and sesamolin in sesame oil samples were quantitatively determined by high performance liquid chromatog- raphy with Hibar RT250-4 column, using a mixture of CH4O-H2O(75:25, V/V) as mobile phase and DAD at 280nm.Good linear relationship was acquired within the range of 10～100μg/ml. The recovery of sesamin was 101.1% and sesamolin 100.2%, and the relative standard deviation was 2.31%(sesamin), 3.05%(sesamolin). The lowest detection limit was 1.5μg/ml(sesamin), 2.5μg/ml (sesamolin)in the condition of S/N=3(according to the concentration of the sample). The content range of sesamin was 0.35%～ 0.72%, and the sesamolin was 0.32%～0.48%. The method is rapid, simple, accurate and reproducible.In oil process, the strength of baking affects directly on content of sesamolin.

Key words: sesame, sesamin, sesamolin, HPLC, sdiversification