Study on Microwave-assisted Extraction of Polysaccharides from Spores of Ganoderma atrum with Response Surface Analysis

Abstract

Abstract: To optimize the microwave-assisted extraction(MAE) of polysaccharide from spores of Ganoderma atrum(PSGA), on the base of the single factor investigation, the extraction temperature, extraction duration and the ratio of water to material were studied with the central composite design and their interactions on the yield of polysaccharide from spores of Ganoderma atrum were also investigated. The predictive polynomial quadratic equations model was developed by SAS software. Optimal conditions of MAE of polysaccharides from spores of Ganoderma atrum can be concluded as follows: 27 min at 129 ℃, the ratio of solvent to material 31:1, purified water as the solvent. On these conditions, the yield of the PSGA is up to 2.64%.

Key words: spores of Ganoderma atrum, microwave-assisted extraction, polysaccharides, response surface analysis

HUANG Pu, XIE Ming-Yong, NIE Shao-Ping, CHEN Yi, LI Chang, XIE Jian-Hua. Study on Microwave-assisted Extraction of Polysaccharides from Spores of Ganoderma atrum with Response Surface Analysis[J]. FOOD SCIENCE, 2007, 28(10): 200-203.

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Study on Microwave-assisted Extraction of Polysaccharides from Spores of Ganoderma atrum with Response Surface Analysis

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