Abstract: The effects of cultivation conditions such as temperature, intial pH, and broth volume on the bioacitivities of the biotransformation products of EGB by Hericium erinaceus in shaking flask were further investigated. Results showed that the suitable culture temperature was 25～30 ℃, the optimal intial pH for biotransformation process was 5.5～6.5 in culture medium containing 0.5% EGB. Furthermore, adding the 10 g/L CaCO3 could also ehance the activity. The kinetics parameters of the biotransformation process were analyzed in fermenter scale. The best fermentation technology of 20 L fermentation tank were as follow: the aeration volume was 8 L/min before 72 h and 4.8 L/min after 72 h and agitation ration was 120r/min.

Key words: Hericium erinaceus, EGB, non-enzymatic glycation, technology conditions