FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (11): 407-410.

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Determinatin of Chlorogenic Acid in Hawthorn by RP-HPLC

HU  Zhi-Guo, WANG  Yuan-Xing, CHEN  Zhen-Gui, XIE  Ming-Yong, HUANG  You-Sheng   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2007-11-15 Published:2011-11-22

Abstract: In this article, the chemical components of the hawthorn were isolated and the contents of chlorogenic acid in the hawthorn were determined by reversed-phase high-performance liquid chromatography on Kromasil C18 column(5μm,250× 4.6mm)with UV detector, at the wave length of 327 nm, at 35℃. A mixture of water and formic acid and acetronitrile (CH3CN: H2O:HCOOH=9:91:0.1) were used with a flow rate of 0.8 ml/min. The average recovery and relative standard deviation of this method were 98.2% and 0.80%.

Key words: hawthorn, HPLC, chlorogenic acid