Abstract: Static adsorption test and static de-adsorption test were carried out to screen the best macroporous resin from ten kinds of macroporous resins for the dynamic adsorption experiments on polyphenols in apple pomace. The antimicrobial activities of purified apple polyphenols in apple pomace and the effects of the factors including concentration, pH value, temperature on the antimicrobial activities were studied by means of filter paper disks. The results showed that macroporous resin D4020 possessed the strong ad-sorption ability to apple polyphenols among the various resins studied, in addition to the easy de-adsorption property. Apple polyphenols had strong inhibition on bacteria activities but no distinct inhibition on mildew and microzyme. The minimum antimicrobial concentration of the purified apple polyphenols on Escherichia coli and Staphylococcus aureus is 0.015%(W/W). The purified apple polyphenols showed that the strongest antimicrobial activities in pH5～6, and the antimicrobial activities of the purified apple polyphenols would not be affected in a certain temperature range.

Key words: apple pomace, polyphenols, purification, antimicrobial activity