Study on Identification of Endophyte with Glutamate Decarboxylase Activity from Honeysuckle and Its Biotransformation Properties

Abstract

Abstract: An endophytic bacterium strain EJH-7 with glutamate decarboxylase activity, transforming 241.224μmol L- glutamate to GABA in 24h by 1g cell (DW), was isolated and screened from the stem of Lonicera japonica Thunb. Through the morphologic, physiological and biochemical characteritics and the 16S rDNA sequence analysis, EJH-7 has been identified as Bacillus megaterium. Also, the phylogenesis was analyzed through the phylogenetic tree base on the 16S rDNA sequences, indicating that strain EJH-7 belonged to Bacteria, Firmicutes, Bacillales, Bacillaceae, Bacullus. The properties of the biotrans- formation (converting L-glutamate to GABA) of Bacillus megaterium EJH-7 were also examined. As a result, the optimum temperature and pH value for biotransformation are 30℃ and 5.5 respectively. The biotransformation activities are inhibited by ..Tween 20 and Tween 80, and hardly affected by Triton 100. The biotransformation activities of Bacillus megaterium EJH-7 are significantly increased by 2.5mmol/L of Ca2+, Cu2+ and Co2+, and raised by 20.36%, 46.61% and 6.77% respectivelyin different degree by K+, Fe2+, Zn2+, Mg2+ and Mn2+.

Key words: glutamate decarboxylase, honeysuckle, endophyte, Bacillus megaterium, 16s rDNA, biotransformation

YANG Sheng-Yuan, LU Zhao-Xin, SUN Li-Jun, BIE Xiao-Mei, 吕Feng-Xia , ZENG Xiao-Xiong. Study on Identification of Endophyte with Glutamate Decarboxylase Activity from Honeysuckle and Its Biotransformation Properties[J]. FOOD SCIENCE, 2007, 28(2): 196-202.

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Study on Identification of Endophyte with Glutamate Decarboxylase Activity from Honeysuckle and Its Biotransformation Properties

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