Abstract: Objective: To study the adjustment effects of seed oil of Punica gramatum L. on blood-lipid and lipid-hyperoxidation in hyperlipidemia rats. Method: 50 female Wistar rats were divided randomly into 5 groups by body weight: control group, hyperlipidemia model group, seed oil of Punica gramatum L. high and low dose group and simvastatin group. The rats were fed with high-lipid foodstuff except control group, so the intervention tests were carried out. Results: After twelve weeks, the contents of serum and hepar total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol(LDL-C), arteriosclerosis index (AI) and malondialdehyde(MDA) of hyperlipidemia model group were all higher than the control group (p<0.05 or p<0.01), but high density lipoprotein ratio to total cholesterol (HDL-C/TC) and superoxide dismutase (SOD) activities were lower than the control group(p<0.05 or p<0.01). The contents of serum and hepar total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol(LDL-C), arteriosclerosis index(AI), malondialdehyde(MDA) in Punica gramatum L. low dose seed oil group were all lower than the hyperlipidemia model group (p<0.05 or p<0.01), but the high density lipoprotein ratio total cholesterol (HDL-C/TC) and superoxide dismutase(SOD) activities were higher than the control hyperlipidemia model group (p<0.05 or p<0.01). Conclusion: Seed oil of Punica gramatum L. has significant effects on controlling hyperlipidemia and atherosclerosisi n rats.

Key words: seed oil of Punica gramatum L., hyperlipidemia rat, lipid peroxidation, prevention