FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (3): 175-178.

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Optimization of Agar Diffusion Bioassay for Nisin

 ZHANG  Guo-Zhi, CHEN  Lin-Hai, YANG  Tian-You, CHEN  Hong-Wei, WANG  Li-Min, DUAN  Yu-Hang, QIN  Guang-Yong   

  1. Ion Beam Bioengineering Provincial Key Lab, Zhengzhou University, Zhengzhou 450052, China
  • Online:2007-03-15 Published:2011-12-31

Abstract: The four major factors agar, Tween20, culture temperature and pre-diffusion, affecting the agar diffusion bioassay for nisin were studied. The results showed that when agar concentration is 0.75%, the addition of Tween20 0.5%, and the culture temperature 30℃, the accuracy and sensitivity of agar bioassay for nisin can be improved. And the pre-diffusion at 4℃ can enhance the diffusion of nisin in agar.

Key words: nisin, agar diffusion bioassay, pre-diffusion