FOOD SCIENCE

Previous Articles     Next Articles

Development of a Magnetic Particle-Based Enzyme-Linked Immunosorbent Assay for Determining Fumonisin B1 in Corn

GUAN Di1,2,3, PAN Can-ping3, WANG Wen1,2, WU Hui-juan1,2,*   

  1. 1. Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China; 2. Beijing Engineering Research Center of Food
    Safety Analysis, Beijing 100089, China; 3. College of Science, China Agricultural University, Beijing 100094, China
  • Online:2014-04-25 Published:2014-05-13
  • Contact: WU Hui-juan

Abstract:

Magnetic particles (MPs) were used as the support to replace enzyme-linked immunosorbent assay (ELISA) plates for
the determination of fumonisin B1 (FB1) in corn. Goat anti-rabbit antibody and protein A-MP conjugates were utilized in a direct
competitive ELISA format. Under optimized conditions, the limits of detection (LOD) for goat anti-rabbit antibody and protein
A-MPs were 0.024 and 0.030 μg/mL, respectively. The goat anti-rabbit antibody MP conjugates exhibited a lower LOD which
was only one third of that of conventional ELISA. The recoveries from spiked corn varied from 76.4% to 110.6%, with relative
standard deviation (RSD) less than 11%. Overall, a sensitive and stable method for FB1 has been developed in this paper.

Key words: magnetic particles, enzyme-linked immunosorbent assay, fumonisins, corn, protein A

CLC Number: