Abstract: Cultivating Hela and LiBr assays in vitro were used to inhibit tumor with about 99% purity lycopene crystals test samples.The results of MTT（methy lthiazolydiphenyl-tetrazolium bromide）,cell growing curve and plate cloning showed that the lycopene crystals inhibition on Hela and LiBr was prominent p＜0.01.IC50were 10.2μmol/L and 10.8μmol/L,respectively. The dose-effects relationship and time-effects relationship were prominent.Cell colony of Hela was not found after 10 d cultivation.And a few cell’s colonies were found in both Libr and LDH cell fluids was increased prominently after 48h.The cell membranes of Hela and LiBr were damaged by lycopene crystals.It indicated that the inhibition on Hela and LiBr cells of lycopene has direct killing rerlationship.

Key words: lycopene, cell cultivating, MTT, antitumor, activity