FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (5): 219-223.

Previous Articles     Next Articles

Research on Aspergillus oryzae Screening with Protoplast Electro Fusion

 TANG  Jie, CHE  Zhen-Ming, LI  Shuan-Mei, ZHAO  Xu-Zhuang   

  1. Bioengineering School,Xihua University,Chengdu 610039,China
  • Online:2007-05-15 Published:2011-12-31

Abstract: This experiment inactivated protoplast of Aspergillus oryzae AS3.951 and CICC2339 with ultraviolet.Then the inactivated protoplast were fused with electro fusion.At last,two fused strains are screened namely EF113 and EF 222.Both of them shows high activity reaching 7680U/g and 7200U/g respectively,much higher than parent strain AS3.951.

Key words: Aspergillus oryzae, protoplast, ultraviolet inactivated, electro fusion