FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (8): 205-208.

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Study on Purification and Antioxidation of Water-soluble Polysaccharide Isolated from Semen Cassia

 GUO  Xiao-Qiang, YAN  Jun, WU  Xiao-Yong, XU  Guang-Yu, FAN  Chun-Hua, GOU  Xiao-Jun   

  1. 1.Laboratory of Traditional Chinese Medicine, Chengdu University, Chengdu 610106, China; 2.Key Laboratory of Medical Microbial Resources, Chengdu University, Chengdu 610106, China
  • Online:2007-08-15 Published:2011-10-24

Abstract: In this paper, Semen Cassia crude polysaccharide is got by water-extracting and alcohol precipitating, then being refined on the two methods of H2O2 bleaching and secondary alcohol precipitation, H2O2 bleaching can effectively remove protein impurities among Semen Cassia crude polysaccharide. Semen Cassia polysaccharide antioxidant experiments show: It is certain to eliminate hydroxyl radical (·OH) by Semen Cassia polysaccharide; and Semen Cassia polysaccharide inhibite effectly superoxide anion radical (O2·) by pyrogallol autoxidation with concentration of 0.022 mg/ml.

Key words: Semen Cassia polysaccharide, purification, anti-oxidation