Study on Fermentation of Recombinant Strain E.coli BL21 /pET-DsbA-MalQ to Produce Amylomaltase

Abstract

Abstract: The effect of inducing time, inducing temperature, concentration of IPTG and concentration of cells (OD600) on fermentation of E.coli BL21/pET-DsbA-MalQ were studied. The optimum conditions were inducing time 2.5 h, inducing temperature 30 ℃, concentration of IPTG 0.5 mmol/L and concentration of cells (OD600) 0.5 respectively. When the E.coli BL21/pET-DsbA-MalQ was incubated at the optimum conditions, the activity of crude enzyme was 130 U, and it was 135 times of enzyme activity of initial strain.

Key words: , E.coli BL21/pET-DsbA-MalQ； inducing time； inducing temperature； concentration of IPTG； amylomaltase；

WANG Shui-Xing, LI Yan-Ping, XU Yang, XIA Hui-Ling, WU Ling-Wei, YU Zhen-Jun. Study on Fermentation of Recombinant Strain E.coli BL21 /pET-DsbA-MalQ to Produce Amylomaltase[J]. FOOD SCIENCE, 2007, 28(8): 285-289.

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Study on Fermentation of Recombinant Strain E.coli BL21 /pET-DsbA-MalQ to Produce Amylomaltase

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