Abstract: Effects of trace element iron and zinc on amino acids in alfalfa sprouts were studied in the paper. With the method of non- earth cultivation, the alfalfa bud is raised for 7 days in the artificial climate box with temperature 25℃, and humidity 75 percent, and watered with the ferrous sulfate and the zinc sulfate solution 600 mg/kg concentration. The contents of amino acids in the above alfalfa sprouts bud were determinated with HPLC. Results showed that the contents of the 17 kinds of amino acids all increase except Asp, Lys, Tyr. Zinc has relatively stronger effects on the amino acids contents in alfalfa, and excessive amount of iron can reduce the absorption and the utilization of zinc in alfalfa sprouts.

Key words: alfalfa sprouts, ferrous sulfate, zinc sulfate, amino acid, HPLC