FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (9): 283-286.

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Optimization of Extraction Technology of Chondroitin Sulfate from Eggshell Membrane

 LIU  Guo-Qing, LING  Qing-Zhi, SUN  Jun-Fei   

  1. School of Biotechnology and Food Engineering,Hefei University of Technology,Heifei 230009,China
  • Online:2007-09-15 Published:2011-11-22

Abstract: Chondroitin sulfate was extracted from the eggshell membrane with a method of dilute alkali-enzyme hydrolysis.The product of chondroitin sulfate was gotten after ethanol disposition and desiccation by eliminating the protein with trypsin and pepsin.The results showed the optimum conditions of weight proportion 1:1.5(material:liquid),alkali concentration 5%,temperature 40 ℃.Dosage of trypsin and pepsin 1.5% and 0.8% respectively,pH value 8.2 and 5.5 respectively and reaction time 2.0 h respectively.

Key words: chondroitin sulfate, eggshell membrane, trypsin, pepsin