FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (9): 442-445.

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Separation,Identification and Determination of Dilinoleoyl-phosphatidylcholine by HPLC-TLC/HPLC-MS

 CAO  Dong   

  1. School of Food Science and Technology,Jiangnan University,Wuxi 214122,China
  • Online:2007-09-15 Published:2011-11-22

Abstract: The analytical method of dilinoleoyl-phosphatidylcholine was studied in this paper.The phosphatidylcholine was separated out from phospholipid by HPLC.The dilinoleoyl-phosphatidylcholine was futher separated out from the phosphati-dylcholine by another HPLC system.Qualitative analysis of dilinoleoyl-phosphatidylcholine was completed with the aid of standard sample and MS(mass spectrum).Finally quantitative analysis of dilinoleoyl-phosphatidylcholine was completed with the aid of standard sample.It was known by tests of method reclamation and precision that the method is accurate,reliable,and handy in the analysis of dilinoleoyl-phosphatidylcholine from phospholipid or phosphatidyl-choline.

Key words: dilinoleoyl-phosphatidylcholine, phosphatidylcholine, HPLC