Quantitative Analysis of Isoflavones in Heshui Pueraria thomsonii by Ultraviolet Spectrophotometry and HPLC

Abstract

Abstract: This study aimed to establish the methods for quantitative analysis of isoflavones of Heshui Pueraria thomsonii. with puerarin as standard,the quantitative analysis of isoflavones in the ethanol extract from Heshui Pueraria thomsonii was conducted by ultraviolet (UV) spectrophotometry and HPLC. The results showed that higher recovery (99.4 %) of the HPLC method was achieved under the conditions of mixture of methanol and water (60:40,V/V) plus 0.25 % acetic acid as mobile phase,flow rate 1.0 ml/min and UV detection wavelength 250 nm,and the recovery of the UV method is also higher (98.7 %),but the determination results of the isoflavones content by this method are higher than those determined by HPLC.

Key words: Heshui Pueraria thomsoni, iisoflavones, ultraviolet spectrophotometry, HPLC

DONG Hua-Qiang, LIU Fu-Lai, LIN Li-Chao, HUANG Jian-Bo, LI Mei. Quantitative Analysis of Isoflavones in Heshui Pueraria thomsonii by Ultraviolet Spectrophotometry and HPLC[J]. FOOD SCIENCE, 2008, 29(10): 477-479.

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Quantitative Analysis of Isoflavones in Heshui Pueraria thomsonii by Ultraviolet Spectrophotometry and HPLC

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