Detection of Laccase Activity and Optimization of Conditions on Laccase Production by Ganoderma lucidum

Abstract

Abstract: A rapid and stable method for laccase assay in Ganoderma lucidum was screened from several different methods: the activity of laccase was detected by adding 0.04% guaiacol to the minimum medium and assaying the diameters of colored rings; Meanwhile, the optimal conditions for laccase production were obtained by the means of single factor analysis and orthogonal method: maltose 3%, yeast extract 0.25%, pH value 6.0, Cu2+ 0.5 mmol/L, culture temperature 25 ℃. These results can provide some references for the breeding of G.lucidum and industrialization of its laccase production.

Key words: Ganoderma lucidum, laccase assay, single factor analysis, orthogonal test

TAO Jun, MA Ai-Min, GUO Ai-Ling, WANG Hui-Jie, ZENG Xing-Bo. Detection of Laccase Activity and Optimization of Conditions on Laccase Production by Ganoderma lucidum[J]. FOOD SCIENCE, 2008, 29(3): 310-313.

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Detection of Laccase Activity and Optimization of Conditions on Laccase Production by Ganoderma lucidum

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