FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (3): 350-354.

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Mutation and Characteristics of Low Temperature Resistant Mutants of Spirulina maxima

HAN  Lan, WANG  Yu-Wen, LI  Jian-Hong, LIU  Hai-Yan, CHEN  Ming, WENG  Yong-Ping   

  1. College of Life Sciences, Nanjing Normal University, Nanjing 210097, China
  • Online:2008-03-15 Published:2011-08-24

Abstract: To obtain low-temperature resistant mutant strains, Spirulina maxima was induced by ultraviolet irradiation as a mutagen. Low temperature resistant mutants were screened under low temperature culturing. The mutants YB01 and YB02 grew significantly faster than their parental strain under low temperature 10 ℃, 15 ℃ or 20 ℃ respectively. Furthermore, the contents of carotene and polyunsaturated fatty acids of the mutants were higher than their parental strain obviously. Investigation on physiological characteristics of mutant YB02 showed that photosynthetic oxygen evolution rate and proline content are higher than their parental strain. To improve mutant growth, the daughter mutant YB02 are also mutated twice. Two granddaughter mutants YB0201and YB0202 are also obtained. Their growth is faster than YB02.

Key words: Spirulina maxima, ultraviolet induce, low temperature, unsaturated fatty acid