Isolation and Identification of Two Major 5-Hydroxytryptamine Derivatives from Safflower Seeds(Carthamus tinctorius L.)

Abstract

Abstract: The isolation and HPLC detection methods of two main 5-hydroxytryptamine(5-HT) derivatives contained in safflower (Carthamus tinctorius L.) oil cake were set up.The total 5-HT derivatives were extracted from safflower oil cake by alcohol. Two compounds were isolated and purified by macroporus resin and C18 columns and identified by HPLC-MS, 1H-NMR and 13C-NMR. The results showed that purity of N-feruloylserotonin(S1) and 1N-(p-coumaroyl)serotonin separately were 96.76% and 90.29% assayed by HPLC-PDA.

Key words: safflower, N-feruloylserotonin, N-(p-coumaroyl)serotonin, HPLC-MS, NMR

YUE Jin-Huan, TAO Guan-Jun, JIN Qing-Zhe, WANG Xing-Guo. Isolation and Identification of Two Major 5-Hydroxytryptamine Derivatives from Safflower Seeds(Carthamus tinctorius L.)[J]. FOOD SCIENCE, 2008, 29(4): 117-120.

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Isolation and Identification of Two Major 5-Hydroxytryptamine Derivatives from Safflower Seeds(Carthamus tinctorius L.)

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